The RBI last week allowed lenders to issue bonds for infrastructure lending, but barred the banks from holding each other's bonds.
"Restriction on cross holding does apply to trading also," Gandhi told Reuters.
Gandhi said the central bank would prefer that these bonds for infrastructure lending attract investors from outside the banking sector.
"The idea is funds to come from outside the banking system," he said.
Dealers had been confused about whether the cross holding restriction also meant that the banks were not allowed to trade in these bonds, given that lenders are crucial market makers in this segment.
"Debt capital market traders in banks will help create liquidity in this market because they are market makers, otherwise liquidity in this segment will not pick up," said a senior dealer at a bank.